White Blood Cell Count with Differential 
Mnemonic WBCD
CPT 85004†
Test Code 2000633
Synonyms WBCD, Leukocyte Count
Test Includes  
Specimen Whole Blood
Volume 4.0 mL
Container Lavender Top (EDTA) Tube
Special Instructions   Invert immediately 8-10 times and mix with anticoagulant.
Specimen Prep Do NOT centrifuge. Invert immediately and mix with anticoagulant.
Storage Room Temperature, preferred or Refrigerate
Transport Temp Room Temperature
Stability Rm Temp 24 hours
Stability Refrig 24 hours
Stability Frozen Unacceptable
Methodology Automated Cell Counter
Rejection Criteria   
Reference Value Adult Normals (>21y):; WBC: 3.9 - 11.2 x 10e3/µL; Differential (includes RBC morphology if present); Standard Differential:; Neutrophil: 43 - 74% (1.9 - 7.8 x 10e3/µL); Lymphocyte: 17 - 46% (0.9 - 3.2 x 10e3/µL); Monocyte: 4 - 13% (0.26 - 0.86 x 10e3/µL); Eosinophil: 0 - 6% (0.3 - 0.52 x 10e3/µL); Basophil: 0 - 1% (0.01 - 0.10 x 10e3/µL); Immature Granulocyte: 0 - 1%; 
Performed/TAT Monday - Sunday        
Comments Automated differential performed in all cases except when significant abnormalities detected. Confirmatory or additional tests may be run with additional charges.