Varicella Zoster Virus Nucleic Acid Amplified Test (NAAT) 
Mnemonic V VZV NAAT
CPT 87798
Test Code 6750060
Synonyms Shell Vial for VZV, VZV rapid culture, rapid viral culture for VZV, Herpes Zoster culture, VZV PCR
Test Includes  
Specimen Bronchial Alveolar Lavage (BAL); Bronchial Wash; CSF; Dermal Lesion or Vesicle; Sputum; Sterile Body Fluid; Tissue
Volume BAL: 5.0 mL; Bronchial Wash: 5.0 mL; Fluid: 3.0 mL; CSF: 1.0 mL; Sputum: 5.0 mL
Container M-4, M-4RT, M-5, or M-6 Viral Transport Medium; Universal Transport Medium (UTM); Liquid Stuart's Medium; Liquid Amies Medium; Sterile Screw Cap Container
Special Instructions BAL or BRONCHIAL WASH: Collect specimen using aseptic technique and accepted methods. Submit 5.0 mL (0.5 mL minimum) in a sterile container without additives. FLUID: Submit 3.0 mL (0.5 mL minimum) in a sterile syringe with air removed. Alternatively, inject specimen into a sterile container without additives. VESICLE SWAB: disrupt vesicle and collect fluid with swab provided with M-4, M-4RT, M-5, M-6 Viral Transport Medium or Universal Transport Medium. With the same swab, collect cells from the base of the lesion. Place swab into M-4, M-4RT, M-5, or M-6 Viral Transport Medium or Universal Transport Medium. VESICLE ASPIRATE: Aspirate fluid from vesicle using a tuberculin syringe. Transfer to M-4 or M-6 Viral Transport Medium. NONVESICULAR LESION: Moisten swab with small amount of sterile saline and collect cells from base of lesion. Place swab into M-4 or M-6 Viral Transport Medium. TISSUE: Grind the tissue in 0.5 mL M4 media following the tissue grinder system procedure. Return the tissue (or portion of tissue) to M4 tube.
Specimen Prep  
Storage Room Temperature of Refrigerated
Transport Temp Room Temperature
Stability Rm Temp 24 hours
Stability Refrig 4 days
Stability Frozen 3 months
Methodology Polymerase Chain Reaction (PCR)
Rejection Criteria Inappropriate specimen type, inadequate volume, inappropriate transport device. 
Reference Value Negative 
Performed/TAT Monday - Friday        
Comments On non-genital dermal specimens, it is recommended to also test for Herpes simplex virus types 1 and 2 (see V HSV NAAT). All new Varicella zoster viruses detected from each non-genital source are called.