Legionella pneumophila/Tatlockia micdadei Nucleic Acid Amplified Test 
Mnemonic LEGIONNAAT
CPT 87541, 87798
Test Code 7000065
Synonyms Atypical pneumonia, PPLO, Pneumoplex 
Test Includes Polymerase Chain Reaction (PCR) for Legionella pneumophila and Tatlockia micdadei in respiratory specimens 
Specimen Bronchial Alveolar Lavage (BAL); Bronchial Wash; Nasal Aspirate; Nasal Wash; Nasopharyngeal; Sputum
Volume BAL: 5.0 mL; Bronchial Wash: 5.0 mL; Sputum: 5.0 mL
Container Red-Capped Bacterial Transport Dual Swab with Liquid Stuart's medium; Blue-Capped Bacterial Transport with Amies Gel medium; Dark Green-Capped Bacterial Transport Mini-tip Swab with Liquid Stuart's medium; Sterile Screw Cap Container
Special Instructions BAL or BRONCHIAL WASH: Collect specimen using aseptic technique and accepted methods. Submit 5.0 mL (0.5 mL minimum) in a sterile container without additives.; NASAL ASPIRATE: Insert a depressed bulb deeply into either nostril. Suction while withdrawing bulb. Transfer aspirate to a sterile container without additives.; NASAL WASH: Aspirate 3.0-5.0 mL sterile saline into rubber bulb. Press one nostril closed. Insert bulb into other nostril. Squeeze saline in and aspirate back into bulb. Transfer to sterile container without additives.; NASOPHARYNGEAL: Insert a flexible mini-tip swab through the nostril into the nasopharynx. Rotate gently and withdraw. Place swab into M-4 or M-6 Viral Transport Medium.; SPUTUM: Have patient cough deeply and collect entire specimen (0.5 mL minimum) of expectorated sputum in a sterile container without additives.
Specimen Prep  
Storage Refrigerate
Transport Temp Room Temperature
Stability Rm Temp 4 days
Stability Refrig 4 days
Stability Frozen  
Methodology Polymerase Chain Reaction (PCR)
Rejection Criteria Inappropriate specimen type, inadequate volume, inappropriate transport device 
Reference Value Negative 
Performed/TAT Three times per week   24-72 hours     
Comments Testing is only performed on the respiratory specimens listed. Testing is not performed on throat specimens. All new positive specimens are called. This test does not distinguish between living and dead organisms and should not be used as a test-of-cure.