JAK2 V617F Mutation Nucleic Acid Amplification Test (NAAT) 
Mnemonic JAK2 NAAT
CPT 81270
Test Code 7000312
Synonyms Janus Kinase 2, JAK2
Test Includes  
Specimen Whole Blood: Bone Marrow
Volume Blood: 4.0 mL; Bone Marrow: 1.0 mL
Container Blood: Lavender Top (EDTA) tube; Bone Marrow: Heparinized Syringe (remove needle and cap syringe or transfer to a sterile screw capped tube)
Special Instructions Deliver DEDICATED Lavender Top tube, UNCENTRIFUGED, to Microbiology.
Specimen Prep  
Storage Refrigerated
Transport Temp Room Temperature
Stability Rm Temp 7 days
Stability Refrig 7 days
Stability Frozen 7 days
Methodology Polymerase Chain Reaction (PCR)
Rejection Criteria Inadequate volume, inappropriate collection tube, centrifuged specimen, inappropriate transport conditions, leaking specimen, delay in transport. 
Reference Value Negative 
Performed/TAT Two times per week   72 hours    
Comments Lower limit of detection is 1% V617F JAK2 DNA. with an accuracy of >99%.