D Aminolevulinic Acid, Urine 24-Hour 
Mnemonic U ALA T
CPT 82135
Test Code 3502692
Synonyms ALA, Delta-ALA
Test Includes  
Specimen Urine (24 Hour) Protected From Light, pH<6
Volume 3.0 mL
Container Plastic Urine Container (24 Hour) with 30 mL of 30% Glacial Acetic Acid
Special Instructions Specimen is NOT stable preserved with Sodium Carbonate. Instruct the patient to void at 8 AM and discard the specimen. Then collect all urine including the final specimen voided at the end of the 24-hour collection period (ie, 8 AM the next morning). PROTECT FROM LIGHT and keep refrigerated during collection.
Specimen Prep Specimen pH must be less than 6. FREEZE IMMEDIATELY. Use an amber plastic urine cup and amber cap. If amber cups are unavailable, cover plastic container completely, top and bottom, with aluminum foil. Identify specimen with patient name directly on the container and on the outside of the aluminum foil. Secure with tape.
Storage Freeze and Protect from Light
Transport Temp Frozen. Protect From Light.
Stability Rm Temp unacceptable
Stability Refrig With 30% Glacial Acetic Acid: 3 days
Stability Frozen With 30% Glacial Acetic Acid: 1 month
Methodology Column Chromatography/Ehrlich Reagent Spectrophotometry
Rejection Criteria   
Reference Value 0.5 - 5.1 mg/24 hours 
Performed/TAT Three times per week   Reported in 3-5 days    
Comments Note 24 hour volume. Total volume performed and charged as needed. Submit separate frozen samples when requesting multiple tests on the same specimen.