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Flow Cytometry

The Wisconsin Diagnostic Laboratories Flow Cytometry Laboratory provides rapid quantitative analysis of lymphocyte subsets and comprehensive qualitative analysis of a broad range of neoplastic disorders of the hematolymphoid system.

Our Medical College of Wisconsin faculty consists of board-certified hematopathologists, including: Horatiu, Olteanu, MD, PhD, Director of Flow Cytometry; Alexandra Harrington, MD, Director of Hematology and Coagulation; Paul Hosking, MD; and Steven Kroft, MD, Vice Chair of Clinical Pathology and Director of Hematopathology.  This group maintains nationally recognized expertise in flow cytometric analysis of leukemia and lymphoma, and has authored numerous publications in this area.

Our approach to Leukemia/Lymphoma Immunophenotyping:

All specimens are processed by highly trained flow cytometry technologists and triaged by a hematopathologist to one of twenty unique, pre-established antibody panels.
We are one of a limited number of laboratories utilizing leading edge eight-color (ten parameter) flow cytometry. More information is obtained from a significantly fewer number of cells as compared to standard four-color (six parameter) flow cytometry.
Custom designed panels are available, depending on suspected pathology or other unique clinical circumstances.
Eight color (ten parameter) data is analyzed by hematopathologists using a powerful flexible approach known as “cluster analysis”,an analytic strategy driven by the characteristics of cell population in the sample.
Preliminary results are available within 1 business day. Final interpretations, rendered by board-certified hematopathologists, represent a synthesis of immunophenotypic, clinical, and morphologic data, and are reported within 48 hours of receipt in the laboratory for the majority (90%) of cases.

Testing Menu

Diagnosis of Hematologic Neoplasias:

Acute Leukemias
Mature B & T Cell Neoplasms
Multiple Myeloma and related disorders
Myeloproliferative and Myelodysplastic disorders

Minimal Residual Disease Monitoring Following Treatment of Hematologic Neoplasms:

Bone Marrow and Solid Organ Transplant Testing:

Immune Reconstitution, including regulatory T-cells (Tregs)

Paroxysmal nocturnal Hemoglobinuria (PNH):

WBC & RBC surface antigens

Immune Status:

HIV monitoring
Quantitation of Lymphocyte Subsets (T-, B-, and NK- cells)
Bronchial Alveolar Lavage (BAL) CD4/CD8 ratio for Sarcoidosis
Rituximab monitoring (CD20 B Cells)
CAMPATH monitoring (CD52)

For further information please contact:
Horatiu Olteanu, MD, PhD
Section Director - Flow Cytometry

Chuck Schur, MT, QCYM
Manager Flow, Cytometry

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